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Mulberry is found in wide variety of climatic, geographic and soil conditions. Mulberry leaf is mainly used to feed the silkworm. As silkworm being the monophagous and solely depends on mulberry leaf. It is distributed in temperate, subtropical and tropical regions with varying varieties to each region. Regional distribution affects the biochemical constituents of leaf. Present study was undertaken to determine the carbohydrate and protein content from three regional varieties of mulberry leaf viz., Botatul (Morus indica), Shahtul (Morus alba) and Chatatul (Morus alba) at three different stages (tender, medium and coarse). In general, decreasing trend in terms of total protein and carbohydrate content was recorded from tender to coarse textured leaves of all the three varieties. The leaf extract of Botatul (tender) and Chatatul (tender) recorded the highest protein and carbohydrate content to the tone of 24.67% and 15.76% respectively. Among the varieties, Botatul recorded highest protein content of 22.42%, whereas highest carbohydrate content (14.98%) was found in Chatatul mulberry variety.
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